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INFORMATION:

VALIDATION PROTOCOL FOR HPV IN SITU HYBRIDIZATION  

There has been a recent strong push to incorporate HPV testing into the diagnostic surgical and cytopathology laboratory.  This is especially true for biopsies and Pap smears that contain atypical squamous cells of undetermined significance.  HPV testing by in situ hybridization has many advantages over hybrid capture or PCR, including the ability to actually see the infected cells and to detect ONLY productive infection.  In situ HPV testing is now automated; the attached document details a validation protocol for HPV using the BenchMark System of Ventana.

To view the documentation in Microsoft

Power Point format click the link below.

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so please be patient. To advance thru the presentation

simply left click.

Click here for Documentation

TEXTBOOKS:

  • Nuovo, Gerard J, PCR in situ Hybridization: Protocols and Applications, 1997, Lippincott Williams & Wilkins Press, 3rd edition.
  • To order PCR in situ Hybridization: Protocols and Applications, directly from Lippencott Williams & Wilkins Press (click on the link).
  • To order Dr. Nuovo's step by step instructional video "Key to successful in situ PCR" directly from Lippencott Williams & Wilkins Press (click on the link).

ARTICLES:

  • Below is a list of some of the articles available on the internet (click on the link) that were authored in whole or in part by Dr. Gerard J. Nuovo:

"The Foundation of Successful RT in situ PCR"

"In situ detection of PCR-amplified metalloproteinase cDNAs, their inhibitors and human papillomavirus transcripts in cervical carcinoma cell lines."

Int J Cancer 71(6), 1056-1060 (1997)

"The foundations of successful RT in situ PCR."

Front Biosci 1, C4-C15 (1996)

"AIDS dementia is associated with massive, activated HIV-1 infection and concomitant expression of several cytokines."

Mol Med 2(3), 358-366 (1996)

"Analysis of fatal pulmonary hantaviral infection in New York by reverse transcriptase in situ polymerase chain reaction."

Am J Pathol 148(3), 685-692 (1996)

"Detection of viral infections by in situ PCR: theoretical considerations and possible value in diagnostic pathology."

J Clin Lab Anal 10(6), 335-349 (1996)

"PCR-amplified vitronectin mRNA localizes in situ to spermatocytes and round spermatids in the human testis."

Hum Reprod 10(8), 2187-2191 (1995)

"In situ PCR: protocols and applications."

PCR Methods Appl 4(4), S151-S167 (1995)

"Correlation of the in situ detection of polymerase chain reaction-amplified metalloproteinase complementary DNAs and their inhibitors with prognosis in cervical carcinoma."

Cancer Res 55(2), 267-275 (1995)

"Analysis of nonspecific DNA synthesis during in situ PCR and solution-phase PCR."

PCR Methods Appl 4(2), 89-96 (1994)

"In situ detection of PCR-amplified HIV-1 nucleic acids in lymph nodes and peripheral blood in patients with asymptomatic HIV-1 infection and advanced-stage AIDS."

J Acquir Immune Defic Syndr 7(9), 916-923 (1994)

MORE:

  • Below is a list of some of the more noteworthy papers, published in major medical journals, authored in whole or in part by Dr. Gerard J. Nuovo:
  1. Nuovo GJ et al. J Virol 1988, 62:1452-1455 (HPV).
  2. Nuovo GJ. JAMA 1990, 263:1223-1226 (HPV).
  3. Nuovo GJ et al. Am J Pathol 1991, 139:847-854(HPV, in situ PCR).
  4. Nuovo GJ et al. Cancer Research, 1995, 55:267-275 (oncogenesis).
  5. Nuovo GJ (co-author). J Virol 1995, 69:32-38 (hepatitus C).
  6. Nuovo GJ (co-author). Proc Natl Acad Sci, USA, 1996 93:700-4 (HIV).
  7. Nuovo GJ. Molecul Med 1996, 2:358-366 (HPV).
  8. Nuovo GJ (co-author). J Clin Invest 1997, 99:1478-83 (oncogenesis).
  9. Nuovo GJ (co-author). J Virol 1997, 71:4079-85 (HIV).
  10. Nuovo GJ (co-author). Virol 1997, 232:379-384(HIV).